6VPB
A novel membrane-bound 6-phosphogluconate dehydrogenase from the acetic acid bacteria Gluconacetobacter diazotrophicus (Gd6PGD)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | RIGAKU MICROMAX-007 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-05-11 |
Detector | DECTRIS PILATUS 200K |
Wavelength(s) | 1.54 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 91.760, 82.097, 81.831 |
Unit cell angles | 90.00, 103.41, 90.00 |
Refinement procedure
Resolution | 36.480 - 1.870 |
R-factor | 0.1838 |
Rwork | 0.183 |
R-free | 0.22220 |
Structure solution method | SAD |
RMSD bond length | 0.009 |
RMSD bond angle | 1.007 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | AutoSol |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 36.480 |
High resolution limit [Å] | 1.870 |
Rmeas | 0.097 |
Rpim | 0.023 |
Number of reflections | 48489 |
<I/σ(I)> | 51.5 |
Completeness [%] | 99.2 |
Redundancy | 16.5 |
CC(1/2) | 0.953 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 291.15 | 10 mM sodium phosphate pH 6.0, 0.1 % triton X-100 |