6VP4
Ethylene forming enzyme (EFE) in complex with Fe(II), L-arginine, and 2OG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2018-06-18 |
Detector | RAYONIX MX-300 |
Wavelength(s) | 0.97872 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 49.730, 78.807, 195.905 |
Unit cell angles | 90.00, 92.67, 90.00 |
Refinement procedure
Resolution | 40.770 - 1.830 |
R-factor | 0.1748 |
Rwork | 0.174 |
R-free | 0.19680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5lsq |
RMSD bond length | 0.006 |
RMSD bond angle | 0.732 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX (1.15.2_3472) |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.770 | 1.890 |
High resolution limit [Å] | 1.830 | 1.830 |
Rpim | 0.031 | 0.301 |
Number of reflections | 132130 | 12759 |
<I/σ(I)> | 18.82 | |
Completeness [%] | 98.7 | |
Redundancy | 6.9 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M Bis-Tris propane, 0.1-0.2 M sodium chloride, 24-30% PEG6000 |