6VNA
Pden_1323
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-06-20 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 72.843, 117.044, 59.382 |
| Unit cell angles | 90.00, 99.38, 90.00 |
Refinement procedure
| Resolution | 58.657 - 2.200 |
| Rwork | 0.211 |
| R-free | 0.25870 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | itasser |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.516 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0257) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 61.250 | 2.270 |
| High resolution limit [Å] | 2.200 | 2.200 |
| Number of reflections | 32977 | 1677 |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 99.7 | |
| Redundancy | 2 | |
| CC(1/2) | 0.997 | 0.582 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293 | glycerol, PEG 4000, sodium acetate, Tris |
