6VN4
USP7 IN COMPLEX WITH LIGAND COMPOUND 1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X10SA |
| Synchrotron site | SLS |
| Beamline | X10SA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2016-05-14 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.9999 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.380, 70.715, 78.551 |
| Unit cell angles | 90.00, 93.40, 90.00 |
Refinement procedure
| Resolution | 44.000 - 2.690 |
| R-factor | 0.2443 |
| Rwork | 0.242 |
| R-free | 0.29990 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | In House Model |
| RMSD bond length | 0.015 |
| RMSD bond angle | 1.731 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 78.410 | 2.940 |
| High resolution limit [Å] | 2.690 | 2.690 |
| Rmerge | 0.037 | 0.449 |
| Rmeas | 0.045 | 0.550 |
| Number of reflections | 21844 | 87 |
| <I/σ(I)> | 12.86 | 2.72 |
| Completeness [%] | 94.4 | 98.1 |
| Redundancy | 3 | 3 |
| CC(1/2) | 0.999 | 0.937 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 277 | NULL |
