6VMT

Leishmania major Programmed Cell Death Protein 5 Homolog

Experimental procedure

Experimental method	MAD
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	100
Detector technology	CCD
Collection date	2005-03-11
Detector	ADSC QUANTUM 315
Wavelength(s)	0.95373,0.97962,0.97949
Spacegroup name	H 3 2
Unit cell lengths	115.386, 115.386, 58.628
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	25.296 - 2.501
Rwork	0.222
R-free	0.27270
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6iqo
RMSD bond length	0.010
RMSD bond angle	1.605
Data reduction software	MOSFLM
Data scaling software	Aimless (0.7.4)
Phasing software	MoRDa
Refinement software	REFMAC (5.8.0258)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	25.296	25.296	2.600
High resolution limit [Å]	2.500	9.010	2.500
Rmerge	0.114	0.074	6.236
Rmeas	0.122	0.078	6.752
Rpim	0.040	0.025	2.424
Number of reflections	4643	122	306
<I/σ(I)>	11.3
Completeness [%]	87.8	95.2	53.5
Redundancy	8.9	8.9	6.9
CC(1/2)	0.998	0.999	0.264

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.6	300	1.5 M K2HPO4