6VMF
Crystal structure of the Y766F mutant of GoxA soaked with glycine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-10-26 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 107.857, 92.985, 187.048 |
| Unit cell angles | 90.00, 95.06, 90.00 |
Refinement procedure
| Resolution | 48.421 - 2.240 |
| R-factor | 0.2024 |
| Rwork | 0.202 |
| R-free | 0.25020 |
| Structure solution method | FOURIER SYNTHESIS |
| Starting model (for MR) | 6byw |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 48.421 | 2.280 |
| High resolution limit [Å] | 2.240 | 2.240 |
| Rmerge | 0.121 | 0.641 |
| Rmeas | 0.143 | 0.750 |
| Rpim | 0.074 | 0.384 |
| Number of reflections | 173760 | 8718 |
| <I/σ(I)> | 5.9 | 1.9 |
| Completeness [%] | 98.0 | 99.7 |
| Redundancy | 3.7 | 3.7 |
| CC(1/2) | 0.992 | 0.685 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 7.5 | 293 | Protein at 10 mg/mL was combined in a 1:1 ratio with precipitant solution composed of 23% PEG 3350, 0.1 M HEPES pH 7.5, 0.1 M ammonium sulfate. |
