6VMA
T4H2 T cell receptor bound to HLA-A2 presenting gp100 peptide (ITDQVPFSV)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 22-ID |
Synchrotron site | APS |
Beamline | 22-ID |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-24 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 1.00000 |
Spacegroup name | P 64 |
Unit cell lengths | 129.934, 129.934, 115.412 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 19.950 - 2.750 |
R-factor | 0.1795 |
Rwork | 0.176 |
R-free | 0.21920 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1tvh |
RMSD bond length | 0.003 |
RMSD bond angle | 0.560 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 20.000 | 2.800 |
High resolution limit [Å] | 2.750 | 2.750 |
Rmerge | 0.070 | 1.334 |
Rmeas | 0.074 | 1.409 |
Rpim | 0.024 | 0.452 |
Number of reflections | 28619 | 1407 |
<I/σ(I)> | 32.3 | 1.8 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.8 | 9.6 |
CC(1/2) | 1.000 | 0.632 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 296 | 18% v/v PEG10000, 16% v/v glycerol, 100 mM Tris pH 8.5, 100 mM sodium chloride |