6VM5

Structure of Moraxella osloensis Cap4 SAVED/CARF-domain containing receptor

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.2.2
Synchrotron site	ALS
Beamline	8.2.2
Temperature [K]	80
Detector technology	CCD
Collection date	2019-05-24
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.99998
Spacegroup name	P 32 2 1
Unit cell lengths	157.593, 157.593, 63.318
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.360 - 2.350
R-factor	0.208
Rwork	0.207
R-free	0.23360
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	unpublished Se-Met version of the same protein determied by SAD phasing
RMSD bond length	0.006
RMSD bond angle	0.710
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	SOLVE
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.360	2.430
High resolution limit [Å]	2.340	2.340
Rpim	0.032	0.895
Number of reflections	37860	3455
<I/σ(I)>	15.3
Completeness [%]	99.2	92.4
Redundancy	11.2	10.6
CC(1/2)	0.999	0.413

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8.5	291	110 mM Tris-HCl pH 8.5, 21% PEG-400