6VLW
Crystal Structure of 426cOD in Complex with VRC01 Fab
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 5.0.1 |
Synchrotron site | ALS |
Beamline | 5.0.1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-04-25 |
Detector | DECTRIS PILATUS 6M |
Wavelength(s) | 1 |
Spacegroup name | P 43 |
Unit cell lengths | 137.367, 137.367, 66.188 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 48.570 - 3.420 |
R-factor | 0.2128 |
Rwork | 0.210 |
R-free | 0.26100 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5ifa |
RMSD bond length | 0.004 |
RMSD bond angle | 0.663 |
Data reduction software | HKL-2000 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 3.480 |
High resolution limit [Å] | 3.420 | 3.420 |
Number of reflections | 15995 | 854 |
<I/σ(I)> | 25.57 | |
Completeness [%] | 100.0 | |
Redundancy | 6.7 | |
CC(1/2) | 0.992 | 0.661 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 18.425% PEG 3350, 11.011% 2-Methyl-2,4-pentanediol, 0.22M Lithium Sulfate, 0.11M Imidazole pH 6.5 |