6VLN
Crystal structure of 4498 Fab in complex with circumsporozoite protein DND3 and anti-Kappa VHH domain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 23-ID-D |
Synchrotron site | APS |
Beamline | 23-ID-D |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2019-11-07 |
Detector | DECTRIS PILATUS3 6M |
Wavelength(s) | 1.033190 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 62.204, 76.481, 63.693 |
Unit cell angles | 90.00, 98.76, 90.00 |
Refinement procedure
Resolution | 38.240 - 1.630 |
R-factor | 0.2101 |
Rwork | 0.209 |
R-free | 0.23430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6d01 |
RMSD bond length | 0.013 |
RMSD bond angle | 1.234 |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 1.650 |
High resolution limit [Å] | 1.630 | 1.630 |
Number of reflections | 73553 | 2626 |
<I/σ(I)> | 14.6 | |
Completeness [%] | 99.9 | |
Redundancy | 6.7 | |
CC(1/2) | 0.990 | 0.830 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 0.1 M phosphate-citrate pH 4.2, 20% (w/v) polyethylene glycol 8000, 0.2 M sodium chloride |