6VLF

Crystal structure of mouse alpha 1,6-fucosyltransferase, FUT8 in its Apo-form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-08-25
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9537
Spacegroup name	C 1 2 1
Unit cell lengths	184.874, 71.353, 126.951
Unit cell angles	90.00, 126.08, 90.00

Refinement procedure

Resolution	44.527 - 1.800
R-factor	0.1854
Rwork	0.184
R-free	0.20820
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2de0
Data reduction software	XDS
Data scaling software	Aimless (0.7.2)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.200	1.830
High resolution limit [Å]	1.800	1.800
Rmerge	0.052	1.268
Rmeas	0.062	1.496
Rpim	0.033	0.788
Total number of observations	422751	21511
Number of reflections	123197	6122
<I/σ(I)>	10.6	0.9
Completeness [%]	99.6	99.7
Redundancy	3.4	3.5
CC(1/2)	0.999	0.463

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.4	298	12% PEG 3350, 0.25 M NH4SO4, 0.1M Tris, pH 7.4