6VLE

Crystal structure of human alpha 1,6-fucosyltransferase, FUT8 in its Apo-form

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	AUSTRALIAN SYNCHROTRON BEAMLINE MX2
Synchrotron site	Australian Synchrotron
Beamline	MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-09-22
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.9536
Spacegroup name	P 1 21 1
Unit cell lengths	95.019, 62.204, 109.905
Unit cell angles	90.00, 90.88, 90.00

Refinement procedure

Resolution	47.251 - 2.280
R-factor	0.2035
Rwork	0.202
R-free	0.23400
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2de0
Data reduction software	XDS
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.16_3549)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	47.500	2.340
High resolution limit [Å]	2.280	2.280
Rmerge	0.135	1.711
Rmeas	0.151	1.907
Rpim	0.067	0.833
Total number of observations	293792	23265
Number of reflections	58867	4549
<I/σ(I)>	8.2	1
Completeness [%]	99.8	100
Redundancy	5	5.1
CC(1/2)	0.997	0.630

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	298	12% PEG 20000, 0.1 M HEPES, pH 7.5