6VL0
Crystal Structure of the N-prenyltransferase DabA in Complex with GSPP and Mn2+
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | ALS BEAMLINE 8.2.1 |
Synchrotron site | ALS |
Beamline | 8.2.1 |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-08-14 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 1.00 |
Spacegroup name | P 43 21 2 |
Unit cell lengths | 124.237, 124.237, 114.078 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 40.991 - 2.200 |
R-factor | 0.1779 |
Rwork | 0.177 |
R-free | 0.20270 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | autoPROC |
Phasing software | AutoSol |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.951 | 2.207 |
High resolution limit [Å] | 2.200 | 2.200 |
Rmerge | 0.132 | 1.133 |
Rpim | 0.046 | 0.397 |
Number of reflections | 41688 | 466 |
<I/σ(I)> | 15.8 | 3.1 |
Completeness [%] | 90.9 | 100 |
Redundancy | 9.4 | |
CC(1/2) | 0.999 | 0.807 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 282 | 1.5 M sodium citrate 0.05 M HEPES pH 7.5 4 mg/mL DabA was preincubated with 2 mM GSPP, 5 mM MnCl2, and 50 mM L-Glu |