6VK7
Crystal Structure of reduced Methylosinus trichosporium OB3b Soluble Methane Monooxygenase Hydroxylase
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-E |
Synchrotron site | APS |
Beamline | 24-ID-E |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-07-25 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.9792 |
Spacegroup name | C 2 2 21 |
Unit cell lengths | 62.458, 290.452, 139.220 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 49.110 - 2.120 |
R-factor | 0.1644 |
Rwork | 0.162 |
R-free | 0.20330 |
Structure solution method | FOURIER SYNTHESIS |
Starting model (for MR) | 1mhz |
RMSD bond length | 0.006 |
RMSD bond angle | 0.773 |
Data reduction software | XDS |
Data scaling software | PHENIX |
Phasing software | PHENIX |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.110 | 2.160 |
High resolution limit [Å] | 2.120 | 2.120 |
Rmerge | 0.161 | |
Rpim | 0.983 | |
Number of reflections | 72012 | 4337 |
<I/σ(I)> | 6.7 | |
Completeness [%] | 99.5 | |
Redundancy | 5.1 | |
CC(1/2) | 0.994 | 0.401 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | Tacsimate and PEG3350 |