6VJN

Structure of NHP D11A.B5Fab in complex with 16055 V2b peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2017-08-17
Detector	ADSC QUANTUM 315
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	64.780, 74.780, 101.450
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.980 - 2.000
R-factor	0.1829
Rwork	0.180
R-free	0.23640
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6rcs
RMSD bond length	0.011
RMSD bond angle	1.107
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.14_3260)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	48.960	2.050
High resolution limit [Å]	2.000	2.000
Number of reflections	33842	2389
<I/σ(I)>	7.4
Completeness [%]	99.4
Redundancy	6.4
CC(1/2)	0.993	0.863

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	8	293	0.1 M Tris, pH 8.0, 1.32 M sodium/potasssium tartrate