6VJK

Streptavidin mutant M88 (N49C/A86C)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL12-2
Synchrotron site	SSRL
Beamline	BL12-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-04-10
Detector	DECTRIS PILATUS 300K
Wavelength(s)	0.98
Spacegroup name	P 21 21 21
Unit cell lengths	60.260, 79.549, 281.549
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.600
Rwork	0.184
R-free	0.21530
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1swe
RMSD bond length	0.007
RMSD bond angle	1.324
Data reduction software	MOSFLM
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	REFMAC (5.8.0135 2015/10/01)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.001	1.690
High resolution limit [Å]	1.600	1.600
Rmerge	0.133	0.747
Rmeas	0.142	0.793
Rpim	0.048	0.262
Number of reflections	176700	25745
<I/σ(I)>	13	5.4
Completeness [%]	98.7	99.4
Redundancy	8.1	8.1
CC(1/2)	0.994	0.814

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	21% PEG 3350, 8% glycerol, 100 mM Bis-Tris-Cl pH 7.5