6VIL

Crystal structure of mouse BAHCC1 BAH domain in complex with H3K27me3

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 5.0.2
Synchrotron site	ALS
Beamline	5.0.2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-06
Detector	DECTRIS PILATUS3 S 6M
Wavelength(s)	1
Spacegroup name	P 31
Unit cell lengths	184.176, 184.176, 70.276
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	46.044 - 3.301
R-factor	0.2497
Rwork	0.248
R-free	0.28280
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4dov
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	3.420
High resolution limit [Å]	3.300	7.100	3.300
Rmerge	0.190	0.047	1.354
Rmeas	0.218	0.054	1.559
Rpim	0.107	0.026	0.766
Total number of observations	162176
Number of reflections	40066	4008	3995
<I/σ(I)>	3.2
Completeness [%]	99.9	100	100
Redundancy	4	4.1	4
CC(1/2)		0.998	0.297

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	5.4	277	1.6-1.8 M NaH2PO4/K2HPO4