6VIK

Crystal structure of mouse xm RABL3 in complex with GTPgammsS

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2019-10-17
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.979
Spacegroup name	P 65 2 2
Unit cell lengths	61.117, 61.117, 224.311
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	47.866 - 1.700
R-factor	0.2094
Rwork	0.207
R-free	0.23480
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.126	0.041	5.362
Rmeas	0.128	0.041	5.483
Rpim	0.022	0.007	1.130
Total number of observations	960436
Number of reflections	28484	1665	1357
<I/σ(I)>	6.3
Completeness [%]	100.0	99.7	99.9
Redundancy	33.7	31.9	23.4
CC(1/2)		0.998	0.568

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.1 M Sodium citrate tribasic dihydrate pH 5.0, 16% EG 1000