6VIJ

Crystal structure of mouse RABL3 in complex with GDP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2019-04-18
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.007
Spacegroup name	P 21 21 21
Unit cell lengths	54.910, 78.203, 118.532
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.020 - 1.950
R-factor	0.1955
Rwork	0.194
R-free	0.22700
Structure solution method	SAD
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.980
High resolution limit [Å]	1.947	5.290	1.950
Rmerge	0.079	0.029	2.132
Rmeas	0.081	0.030	2.201
Rpim	0.019	0.007	0.541
Number of reflections	37652	2045	1827
<I/σ(I)>	6.3
Completeness [%]	99.0	97.8	99.3
Redundancy	17.8	17	15.8
CC(1/2)		1.000	0.665

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2 M Magnesium chloride hexahydrate, 12% w/v PEG 3350