6VIH
The ligand-free structure of mouse RABL3
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 19-ID |
Synchrotron site | APS |
Beamline | 19-ID |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-10-17 |
Detector | ADSC QUANTUM 315r |
Wavelength(s) | 0.979 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 58.998, 79.200, 111.473 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 47.313 - 2.993 |
R-factor | 0.2313 |
Rwork | 0.227 |
R-free | 0.27500 |
Structure solution method | SAD |
Data reduction software | HKL-3000 |
Data scaling software | HKL-3000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 3.000 |
High resolution limit [Å] | 2.950 | 8.000 | 2.950 |
Rmerge | 0.095 | 0.075 | 3.711 |
Rmeas | 0.098 | 0.077 | 3.815 |
Rpim | 0.024 | 0.019 | 0.877 |
Total number of observations | 205296 | ||
Number of reflections | 11507 | 655 | 546 |
<I/σ(I)> | 7.7 | ||
Completeness [%] | 99.9 | 99.5 | 100 |
Redundancy | 17.8 | 15.1 | 18.7 |
CC(1/2) | 0.996 | 0.517 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 277 | 0.5 M Ammonium phosphate monobasic |