6VHO
Glycoside hydrolase family 16 endo-glucanase from Bacteroides ovatus in complex with G4G4G3G-NHCOCH2Br
This is a non-PDB format compatible entry.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-03-06 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.0332 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 168.580, 61.150, 49.411 |
| Unit cell angles | 90.00, 93.61, 90.00 |
Refinement procedure
| Resolution | 84.123 - 2.148 |
| Rwork | 0.186 |
| R-free | 0.20670 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5nbo |
| RMSD bond length | 0.006 |
| RMSD bond angle | 1.388 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 84.123 | 2.440 |
| High resolution limit [Å] | 2.148 | 2.300 |
| Number of reflections | 27458 | 6716 |
| <I/σ(I)> | 7 | |
| Completeness [%] | 96.0 | |
| Redundancy | 1.8 | |
| CC(1/2) | 0.993 | 0.760 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 0.1 M succinic acid pH 7.2, 15 % (w/v) PEG3350 |
