6VHN

Wild type EGFR in complex with LN2057

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 24-ID-C
Synchrotron site	APS
Beamline	24-ID-C
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-12-11
Detector	DECTRIS EIGER X 9M
Wavelength(s)	0.97918
Spacegroup name	I 2 3
Unit cell lengths	146.865, 146.865, 146.865
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	59.960 - 2.400
R-factor	0.1989
Rwork	0.199
R-free	0.20030
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6d8e
Data reduction software	xia2
Data scaling software	Aimless (0.7.4)
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	59.970	59.970	2.460
High resolution limit [Å]	2.400	10.740	2.400
Rmerge	0.106	0.067	0.683
Rmeas	0.118	0.074	0.757
Rpim	0.049	0.030	0.318
Total number of observations	102763	1275	7659
Number of reflections	20144	239	1483
<I/σ(I)>	8.3	17.1	2.1
Completeness [%]	97.7	93.5	99
Redundancy	5.1	5.3	5.2
CC(1/2)	0.992	0.994	0.720

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	1.4M Sodium Citrate, 0.1 M MES