6VHI
Crystal structure of the human ILRUN Fw domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-07-14 |
| Detector | DECTRIS EIGER X 16M |
| Wavelength(s) | 1.45865 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 135.931, 40.977, 23.291 |
| Unit cell angles | 90.00, 91.43, 90.00 |
Refinement procedure
| Resolution | 67.940 - 2.460 |
| R-factor | 0.1935 |
| Rwork | 0.192 |
| R-free | 0.22170 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4ole |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.110 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | MrBUMP |
| Refinement software | BUSTER (2.10.3) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 67.940 | 2.590 |
| High resolution limit [Å] | 2.460 | 2.460 |
| Rmerge | 0.118 | 0.580 |
| Rmeas | 0.134 | 0.658 |
| Rpim | 0.061 | 0.306 |
| Number of reflections | 4775 | 709 |
| <I/σ(I)> | 7.9 | 2.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 4.5 | 4.5 |
| CC(1/2) | 0.994 | 0.890 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 1 M ammonium sulfate and 0.15 M trisodium citrate-citric acid, pH 5.5 |
