6VH6

Crystal structure of Epstein-Barr Virus Nuclear Antigen-1, EBNA1, bound to fragment

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2015-11-19
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	1.0000
Spacegroup name	P 21 21 21
Unit cell lengths	59.516, 68.344, 69.687
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	21.997 - 1.300
Rwork	0.150
R-free	0.16080
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1vhi
RMSD bond length	0.009
RMSD bond angle	1.007
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (1.17)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.320
High resolution limit [Å]	1.300	3.530	1.300
Rmerge	0.061	0.050	0.800
Rmeas	0.066	0.054	0.946
Number of reflections	69927	3655	3190
<I/σ(I)>	26.4	33.1	1.03
Completeness [%]	99.1	96.3	91.9
Redundancy	6.4	6.5	3.1
CC(1/2)		0.996	0.557

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	298	50 mM MES pH 6.5, 0-100 mM NaCl, 10 mM DTT