6VGW
Crystal structure of VidaL intein (selenomethionine variant)
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | NSLS-II BEAMLINE 17-ID-2 |
Synchrotron site | NSLS-II |
Beamline | 17-ID-2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-10-02 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.979 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 101.486, 40.632, 36.023 |
Unit cell angles | 90.00, 103.55, 90.00 |
Refinement procedure
Resolution | 35.020 - 1.510 |
R-factor | 0.1703 |
Rwork | 0.169 |
R-free | 0.19010 |
Structure solution method | SAD |
RMSD bond length | 0.010 |
RMSD bond angle | 0.879 |
Data reduction software | HKL-3000 |
Data scaling software | Aimless |
Phasing software | SHELXDE |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 35.020 | 1.550 |
High resolution limit [Å] | 1.510 | 1.510 |
Rmerge | 0.072 | 0.689 |
Rmeas | 0.080 | 0.765 |
Rpim | 0.034 | 0.328 |
Number of reflections | 21755 | 1513 |
<I/σ(I)> | 14.3 | 2.3 |
Completeness [%] | 97.2 | 91.3 |
Redundancy | 10.3 | 10 |
CC(1/2) | 0.999 | 0.839 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 4.6 | 293 | 0.1 M sodium acetate (pH 4.6) with 2 M ammonium sulfate |