6VG4
Human protocadherin 10 ectodomain
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2016-08-23 |
Detector | DECTRIS PILATUS 6M-F |
Wavelength(s) | 0.9792 |
Spacegroup name | P 41 21 2 |
Unit cell lengths | 84.087, 84.087, 543.917 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 20.000 - 3.298 |
R-factor | 0.2297 |
Rwork | 0.227 |
R-free | 0.28430 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6vfv 6vfq |
RMSD bond length | 0.010 |
RMSD bond angle | 0.674 |
Data reduction software | XDS (1.10.1_2155) |
Data scaling software | Aimless |
Phasing software | PHASER |
Refinement software | PHENIX (1.10.1_2155) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 40.000 | 3.480 |
High resolution limit [Å] | 3.298 | 3.298 |
Rmerge | 0.139 | 2.475 |
Rmeas | 0.145 | 2.570 |
Rpim | 0.040 | 0.687 |
Number of reflections | 19779 | 4386 |
<I/σ(I)> | 10.7 | 1.6 |
Completeness [%] | 99.7 | 99.5 |
Redundancy | 12.9 | 13.7 |
CC(1/2) | 1.000 | 0.950 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 5 | 296 | 0.22M CaCl2, 0.1M Na Acetate pH 5.0 with 30% (v/v) glycerol as cryoprotectant and glutaraldehyde (0.5% v/v) added to the reservoir after crystal formation to aid crystal stability by cross-linking |