6VFB

DNA Polymerase Mu, 8-oxorGTP:Ct Reaction State Ternary Complex, 50 mM Mn2+ (960 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-07-13
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.198, 62.334, 118.756
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.987 - 1.550
R-factor	0.1676
Rwork	0.167
R-free	0.18150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.580
High resolution limit [Å]	1.545	4.210	1.550
Rmerge	0.101	0.107	0.622
Rmeas	0.111	0.118	0.733
Rpim	0.046	0.049	0.380
Number of reflections	65971	3488	3190
<I/σ(I)>	11.7
Completeness [%]	99.8	97.7	99
Redundancy	5.7	5.5	3.4
CC(1/2)		0.975	0.651

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000