6VF9

DNA Polymerase Mu, 8-oxorGTP:Ct Ternary Complex, 50 mM Mg2+ (2160 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-08-16
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	60.200, 62.130, 118.533
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.928 - 1.560
R-factor	0.1658
Rwork	0.165
R-free	0.18180
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.590
High resolution limit [Å]	1.560	4.230	1.560
Rmerge	0.076	0.056	0.911
Rmeas	0.083	0.062	0.998
Rpim	0.031	0.024	0.399
Total number of observations	426304
Number of reflections	63490	3350	3148
<I/σ(I)>	10.8
Completeness [%]	99.0	96.5	99.7
Redundancy	6.7	6.4	5.9
CC(1/2)		0.990	0.746

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000