6VF5

DNA Polymerase Mu, 8-oxorGTP:At Product State Ternary Complex, 50 mM Mn2+ (120 min)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 22-ID
Synchrotron site	APS
Beamline	22-ID
Temperature [K]	100
Detector technology	PIXEL
Collection date	2018-05-03
Detector	DECTRIS EIGER X 16M
Wavelength(s)	1.00
Spacegroup name	P 21 21 21
Unit cell lengths	59.982, 68.718, 110.413
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	40.620 - 1.600
R-factor	0.1661
Rwork	0.165
R-free	0.19100
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4m04
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHENIX
Refinement software	PHENIX (1.15.2_3472)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	1.630
High resolution limit [Å]	1.600	4.340	1.600
Rmerge	0.073	0.050	0.877
Rmeas	0.082	0.055	0.992
Rpim	0.035	0.024	0.456
Total number of observations	310821
Number of reflections	60365	3145	2953
<I/σ(I)>	12.2
Completeness [%]	99.1	95.8	98.1
Redundancy	5.1	5.1	4.5
CC(1/2)		0.996	0.622

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	7.5	277	85-90mM HEPES pH 7.5, 17-18% PEG4000