6VEM

Structure of RNA octamer

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 21-ID-D
Synchrotron site	APS
Beamline	21-ID-D
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-06-02
Detector	DECTRIS EIGER2 X 9M
Wavelength(s)	0.9183
Spacegroup name	P 61
Unit cell lengths	37.921, 37.921, 57.852
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	21.720 - 1.560
R-factor	0.1799
Rwork	0.177
R-free	0.24390
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	259d
RMSD bond length	0.029
RMSD bond angle	1.857
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP (CCP4)
Refinement software	REFMAC (5.8.0257)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	23.000	1.620
High resolution limit [Å]	1.560	1.560
Rmerge	0.063	0.869
Rpim	0.015	0.243
Number of reflections	6721	627
<I/σ(I)>	63.32	1.33
Completeness [%]	99.3	92.4
Redundancy	18.5	12.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291	Sodium cacodylate (20 mM, pH 6.0), sodium chloride (40 mM), hexamine cobalt(III) chloride (10 mM), and 2-methyl-2,4-pentanediol (MPD; 5% (v/v)