6VEK

Contact-dependent growth inhibition toxin-immunity protein complex from from E. coli 3006, full-length

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	APS BEAMLINE 19-ID
Synchrotron site	APS
Beamline	19-ID
Temperature [K]	100
Detector technology	CCD
Collection date	2016-04-03
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.9793
Spacegroup name	P 21 21 21
Unit cell lengths	41.000, 71.769, 175.538
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	29.256 - 2.250
R-factor	0.1852
Rwork	0.182
R-free	0.23360
Structure solution method	MR-SAD
Starting model (for MR)	6cp8
RMSD bond length	0.007
RMSD bond angle	0.786
Data reduction software	HKL-3000
Data scaling software	HKL-3000
Phasing software	PHENIX
Refinement software	PHENIX (dev_2947)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.000	2.290
High resolution limit [Å]	2.250	2.250
Rmerge	0.132	1.242
Number of reflections	25933	1274
<I/σ(I)>	30.3	1.77
Completeness [%]	99.7	99.7
Redundancy	25.4	17.2
CC(1/2)		0.635

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6.5	277	30% GLYCEROL ETHOXYLATE, 0.2 M AMMONIUM ACETATE, 0.1 M, MES PH 6.5