6VE8
Structure of the Glutamate-Like Receptor GLR3.2 ligand-binding domain in complex with Methionine
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2019-07-08 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.97910 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.645, 65.470, 72.186 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 48.500 - 1.750 |
| R-factor | 0.1667 |
| Rwork | 0.165 |
| R-free | 0.19970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | Homology model based on 6MMS |
| RMSD bond length | 0.012 |
| RMSD bond angle | 1.721 |
| Data reduction software | XDS |
| Data scaling software | Aimless |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0258) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 72.190 | 72.190 | 1.780 |
| High resolution limit [Å] | 1.750 | 9.090 | 1.750 |
| Rmerge | 0.078 | 0.046 | 0.674 |
| Rmeas | 0.087 | 0.051 | 0.806 |
| Rpim | 0.037 | 0.023 | 0.433 |
| Total number of observations | 124336 | ||
| Number of reflections | 23419 | 206 | 1258 |
| <I/σ(I)> | 13.3 | 1.8 | |
| Completeness [%] | 99.8 | 97.2 | 99.1 |
| Redundancy | 5.3 | 4.4 | 3.1 |
| CC(1/2) | 0.998 | 0.998 | 0.655 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | 0.1 M MES pH 6.5, 18% PEG MME 2K, 0.1 M Ammonium sulfate |
