6VDP

Crystal structure of SfmD truncated variant

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRL BEAMLINE BL9-2
Synchrotron site	SSRL
Beamline	BL9-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2017-04-22
Detector	DECTRIS PILATUS3 6M
Wavelength(s)	0.9791
Spacegroup name	P 31 2 1
Unit cell lengths	59.863, 59.863, 155.848
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.190 - 2.000
R-factor	0.2004
Rwork	0.196
R-free	0.24360
Structure solution method	MAD
Data reduction software	DENZO
Data scaling software	SCALEPACK
Phasing software	SOLVE
Refinement software	PHENIX (1.17.1-3660)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.070
High resolution limit [Å]	2.000	4.310	2.000
Rmerge	0.095	0.067	1.689
Rmeas	0.098	0.069	1.739
Rpim	0.022	0.016	0.409
Total number of observations	433971
Number of reflections	22661	2455	2202
<I/σ(I)>	6.9
Completeness [%]	99.9	99.8	99.9
Redundancy	19.2	17.8	17.7
CC(1/2)		0.998	0.753

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		295	0.1 M Tris-HCl pH 8.5, 0.2 M magnesium chloride, 20% PEG 8,000