6VDH
Crystal structure of ancestral apicomplexan lactate dehydrogenase in alternate dimer configuration with sulfate.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 12.3.1 |
| Synchrotron site | ALS |
| Beamline | 12.3.1 |
| Temperature [K] | 90 |
| Detector technology | CCD |
| Collection date | 2019-10-16 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.115866 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 103.380, 105.440, 151.450 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.530 - 1.850 |
| R-factor | 0.2101 |
| Rwork | 0.209 |
| R-free | 0.23620 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4plg |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.592 |
| Data reduction software | XDS |
| Data scaling software | XSCALE |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.17_3644) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.530 | 1.790 |
| High resolution limit [Å] | 1.680 | 1.680 |
| Rmeas | 0.102 | 5.295 |
| Number of reflections | 86980 | 18335 |
| <I/σ(I)> | 3.66 | 0.18 |
| Completeness [%] | 88.9 | 62.6 |
| Redundancy | 5.92 | 3.18 |
| CC(1/2) | 0.998 | 0.071 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 20mg/mL protein, 2mM NADH4; well solution: 5% v/v 2-Propanol, 2.5M Ammonium Sulfate |
