6VDF

Structure of the periplasmic domain of YejM from Salmonella typhimurium (twinned)

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 4.2.2
Synchrotron site	ALS
Beamline	4.2.2
Temperature [K]	80
Detector technology	CMOS
Collection date	2016-06-06
Detector	RDI CMOS_8M
Wavelength(s)	1.001
Spacegroup name	P 1
Unit cell lengths	42.948, 42.957, 181.209
Unit cell angles	94.28, 94.02, 111.78

Refinement procedure

Resolution	59.900 - 1.920
R-factor	0.22
Rwork	0.211
R-free	0.26140
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6vat
Data reduction software	XDS
Data scaling software	Aimless
Phasing software	PHASER
Refinement software	PHENIX (1.17.1_3660)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	59.900	1.989
High resolution limit [Å]	1.920	1.920
Rmerge	0.091	0.762
Rpim		0.758
Number of reflections	70265	5489
<I/σ(I)>	7.3	1.07
Completeness [%]	77.2	60.59
Redundancy	1.6	1.4
CC(1/2)	0.971	0.191

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7	291	0.1 M HEPES pH 7.0, 9 % PEG3350