6VDE
Full-length M. smegmatis Pol1
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 24-ID-C |
Synchrotron site | APS |
Beamline | 24-ID-C |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-02-22 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.97918 |
Spacegroup name | P 1 |
Unit cell lengths | 70.004, 80.940, 117.912 |
Unit cell angles | 98.68, 105.13, 109.36 |
Refinement procedure
Resolution | 50.366 - 2.713 |
R-factor | 0.2368 |
Rwork | 0.234 |
R-free | 0.30420 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6vdd |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.500 | 2.750 |
High resolution limit [Å] | 2.700 | 2.700 |
Rpim | 0.496 | |
Number of reflections | 60140 | 2877 |
<I/σ(I)> | 24 | 1.7 |
Completeness [%] | 98.3 | |
Redundancy | 4.5 | |
CC(1/2) | 0.998 | 0.662 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 1 M lithium chloride, 20% PEG6000, 0.1 M MES, 3% dextran sulfate sodium salt |