6VDE
Full-length M. smegmatis Pol1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 24-ID-C |
| Synchrotron site | APS |
| Beamline | 24-ID-C |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2018-02-22 |
| Detector | DECTRIS PILATUS3 S 6M |
| Wavelength(s) | 0.97918 |
| Spacegroup name | P 1 |
| Unit cell lengths | 70.004, 80.940, 117.912 |
| Unit cell angles | 98.68, 105.13, 109.36 |
Refinement procedure
| Resolution | 50.366 - 2.713 |
| R-factor | 0.2368 |
| Rwork | 0.234 |
| R-free | 0.30420 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 6vdd |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.16_3549) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.500 | 2.750 |
| High resolution limit [Å] | 2.700 | 2.700 |
| Rpim | 0.496 | |
| Number of reflections | 60140 | 2877 |
| <I/σ(I)> | 24 | 1.7 |
| Completeness [%] | 98.3 | |
| Redundancy | 4.5 | |
| CC(1/2) | 0.998 | 0.662 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.5 | 293 | 1 M lithium chloride, 20% PEG6000, 0.1 M MES, 3% dextran sulfate sodium salt |
