6VC8
Crystal structure of wild-type KRAS4b(1-169) in complex with GMPPNP and Mg ion
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-F |
Synchrotron site | APS |
Beamline | 21-ID-F |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2016-03-25 |
Detector | MARMOSAIC 225 mm CCD |
Wavelength(s) | 0.97872 |
Spacegroup name | C 1 2 1 |
Unit cell lengths | 69.210, 82.330, 88.140 |
Unit cell angles | 90.00, 112.92, 90.00 |
Refinement procedure
Resolution | 37.910 - 2.500 |
R-factor | 0.2155 |
Rwork | 0.212 |
R-free | 0.27680 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 3gft |
Data reduction software | XDS |
Data scaling software | XSCALE |
Phasing software | PHASER |
Refinement software | PHENIX (1.17) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 37.910 | 2.560 |
High resolution limit [Å] | 2.500 | 2.500 |
Rmerge | 0.053 | 0.565 |
Number of reflections | 15480 | 1571 |
<I/σ(I)> | 15.5 | 2.02 |
Completeness [%] | 97.3 | 97.3 |
Redundancy | 2.6 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 293 | 32% PEG 4000, 800 mM LiCl, 100 mM Magnesium Chloride and 100 mM Tris pH 8.5 |