6VB3

HLA-B*15:01 complexed with a synthetic peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	CHESS BEAMLINE F1
Synchrotron site	CHESS
Beamline	F1
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-10
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.370800
Spacegroup name	P 21 21 21
Unit cell lengths	50.799, 81.828, 110.453
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	37.388 - 2.000
R-factor	0.2429
Rwork	0.239
R-free	0.30730
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xr8
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	37.390	2.070
High resolution limit [Å]	2.000	2.000
Rmerge		0.121
Rmeas	0.054
Number of reflections	30529	2964
<I/σ(I)>	11
Completeness [%]	95.0
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2 ammonium acetate, 0.M tri-sodium citrate dihydrate pH5.6, 30% w/v PEG4000