6VB0

HLA-B*15:02 complexed with a synthetic peptide

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS-II BEAMLINE 17-ID-2
Synchrotron site	NSLS-II
Beamline	17-ID-2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2019-04-27
Detector	DECTRIS EIGER X 16M
Wavelength(s)	0.92013
Spacegroup name	P 21 21 21
Unit cell lengths	50.821, 81.265, 110.662
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	28.022 - 1.900
R-factor	0.1685
Rwork	0.166
R-free	0.21340
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1xr8
Data reduction software	XDS
Data scaling software	XDS
Phasing software	PHENIX
Refinement software	PHENIX (1.13_2998)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	28.030	1.970
High resolution limit [Å]	1.830	1.890
Rmerge		0.167
Rmeas	0.044
Number of reflections	41129	8079
<I/σ(I)>	14
Completeness [%]	98.9
Redundancy	2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		277	0.2M ammonium acetate, 0.1M tri-sodium citrate dihydrate pH5.6, 30% w/v PEG4000