6VAG
Crystal structure of the oligomerization domain of phosphoprotein from parainfluenza virus 5
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | APS BEAMLINE 21-ID-G |
Synchrotron site | APS |
Beamline | 21-ID-G |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-02-01 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.978 |
Spacegroup name | P 21 21 2 |
Unit cell lengths | 29.893, 36.048, 140.016 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.340 - 1.400 |
R-factor | 0.1559 |
Rwork | 0.155 |
R-free | 0.17970 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | Ideal helices |
RMSD bond length | 0.005 |
RMSD bond angle | 0.693 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.15.2_3472) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.420 |
High resolution limit [Å] | 1.400 | 1.400 |
Rmeas | 0.065 | |
Rpim | 0.025 | |
Number of reflections | 28002 | 1239 |
<I/σ(I)> | 38.12 | 2.08 |
Completeness [%] | 90.8 | |
Redundancy | 6.5 | 7.1 |
CC(1/2) | 0.774 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 6 | 298 | 0.1 M MIB buffer (malonic acid, imidazone, boric acid), pH 6.0 and 25% w/v PEG 1500 |