6V9Z
Cryo-EM structure of PCAT1 bound to its CtA peptide substrate
EM specimen
Vitrification | Yes |
Instrument | FEI VITROBOT MARK IV |
EM Data Collection
Microscope | FEI TITAN KRIOS |
Electron Gun | FIELD EMISSION GUN |
Accelerating Voltage (kV) | 300 |
Detector | GATAN K2 SUMMIT (4k x 4k) |
3D Reconstruction
Method | SINGLE PARTICLE |
Resolution (Å) | 3.35 |
Number of Particle | 572800 |
Software | RELION,cryoSPARC,Coot,Leginon,Gctf,REFMAC |
Symmetry | C1 |
CTF correction | PHASE FLIPPING ONLY |