6V98
Crystal structure of Type VI secretion system effector, TseH (VCA0285)
Experimental procedure
Experimental method | MAD |
Source type | SYNCHROTRON |
Source details | CLSI BEAMLINE 08B1-1 |
Synchrotron site | CLSI |
Beamline | 08B1-1 |
Temperature [K] | 93.15 |
Detector technology | CCD |
Collection date | 2018-01-26 |
Detector | MARMOSAIC 300 mm CCD |
Wavelength(s) | 0.97949 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 45.756, 63.645, 70.292 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 32.850 - 1.800 |
R-factor | 0.1759 |
Rwork | 0.174 |
R-free | 0.21660 |
Structure solution method | MAD |
RMSD bond length | 0.006 |
RMSD bond angle | 0.936 |
Data reduction software | XDS |
Data scaling software | HKL-3000 |
Phasing software | SHARP |
Refinement software | PHENIX (1.17.1_3660) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.830 |
High resolution limit [Å] | 1.800 | 1.800 |
Rmeas | 0.099 | |
Rpim | 0.039 | |
Number of reflections | 19339 | 858 |
<I/σ(I)> | 20 | |
Completeness [%] | 99.1 | |
Redundancy | 6.5 | |
CC(1/2) | 0.691 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | 18% w/v PEG8000, 0.2 M calcium acetate, 0.1 M sodium cacodylate, pH 6.5 |