6V8R

Proteinase K Determined by MicroED Phased by ARCIMBOLDO_SHREDDER

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Wavelength(s)	0.0251
Spacegroup name	P 43 21 2
Unit cell lengths	67.250, 67.250, 99.920
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	55.790 - 1.600
R-factor	0.1985
Rwork	0.195
R-free	0.23170
Structure solution method	MOLECULAR REPLACEMENT
Data reduction software	XDS
Data scaling software	XSCALE
Phasing software	Arcimboldo (ARCIMBOLDO_SHREDDER)
Refinement software	PHENIX

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	55.790	55.790	1.640
High resolution limit [Å]	1.600	7.160	1.600
Rmerge	0.353	0.195	2.709
Rmeas	0.381	0.212	3.026
Total number of observations	194052
Number of reflections	29061	391	1851
<I/σ(I)>	3.31	9.21	0.3
Completeness [%]	93.9	92.9	82.2
Redundancy	6.677	5.877	4.783
CC(1/2)	0.970	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1