6V8Q
Structure of an inner membrane protein required for PhoPQ regulated increases in outer membrane cardiolipin
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 8.2.2 |
| Synchrotron site | ALS |
| Beamline | 8.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 135.831, 64.847, 146.364 |
| Unit cell angles | 90.00, 97.66, 90.00 |
Refinement procedure
| Resolution | 48.342 - 2.700 |
| R-factor | 0.241307653313 |
| Rwork | 0.239 |
| R-free | 0.29114 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | PbgA periplasmic domain |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.573 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.800 |
| High resolution limit [Å] | 2.690 | 2.700 |
| Rpim | 0.086 | |
| Number of reflections | 53782 | 763 |
| <I/σ(I)> | 7.4 | |
| Completeness [%] | 97.6 | |
| Redundancy | 4.3 | |
| CC(1/2) | 0.310 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 298 | 0.4 M (NH4)2SO4, 0.1 M MES (pH 6.5), and 10% (w/v) polyethylene glycol 3350 |
