6V89

Human CtBP1 (28-375) in complex with AMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	Cu FINE FOCUS
Temperature [K]	100
Detector technology	CCD
Collection date	2019-02-22
Detector	RIGAKU SATURN 944
Wavelength(s)	1.54178
Spacegroup name	P 64 2 2
Unit cell lengths	88.944, 88.944, 163.720
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	36.140 - 2.450
R-factor	0.2331
Rwork	0.231
R-free	0.27350
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6cdf
RMSD bond length	0.002
RMSD bond angle	0.466
Data reduction software	HKL-3000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (v1.15)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.490
High resolution limit [Å]	2.450	6.650	2.450
Rmerge	0.095	0.051	0.597
Number of reflections	13982	847	502
<I/σ(I)>	10
Completeness [%]	94.4	96.9	69.6
Redundancy	7.9	9.1	2.5
CC(1/2)	0.998

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	7.5	298	100 mM HEPES, pH 7.5, 80-200 mM calcium chloride, 2-6% PEG400