6V89
Human CtBP1 (28-375) in complex with AMP
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | ROTATING ANODE |
Source details | Cu FINE FOCUS |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2019-02-22 |
Detector | RIGAKU SATURN 944 |
Wavelength(s) | 1.54178 |
Spacegroup name | P 64 2 2 |
Unit cell lengths | 88.944, 88.944, 163.720 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 36.140 - 2.450 |
R-factor | 0.2331 |
Rwork | 0.231 |
R-free | 0.27350 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6cdf |
RMSD bond length | 0.002 |
RMSD bond angle | 0.466 |
Data reduction software | HKL-3000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (v1.15) |
Data quality characteristics
Overall | Inner shell | Outer shell | |
Low resolution limit [Å] | 50.000 | 50.000 | 2.490 |
High resolution limit [Å] | 2.450 | 6.650 | 2.450 |
Rmerge | 0.095 | 0.051 | 0.597 |
Number of reflections | 13982 | 847 | 502 |
<I/σ(I)> | 10 | ||
Completeness [%] | 94.4 | 96.9 | 69.6 |
Redundancy | 7.9 | 9.1 | 2.5 |
CC(1/2) | 0.998 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 298 | 100 mM HEPES, pH 7.5, 80-200 mM calcium chloride, 2-6% PEG400 |