6V81
The crystal structure of the outer-membrane transporter YncD
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
Synchrotron site | Australian Synchrotron |
Beamline | MX2 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2017-04-07 |
Detector | DECTRIS EIGER X 16M |
Wavelength(s) | 0.987 |
Spacegroup name | P 21 2 21 |
Unit cell lengths | 87.940, 98.640, 124.860 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.970 - 2.957 |
R-factor | 0.2789 |
Rwork | 0.277 |
R-free | 0.31420 |
Structure solution method | SAD |
Data reduction software | XDS |
Data scaling software | Aimless |
Phasing software | PHENIX |
Refinement software | PHENIX (1.13_2998) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 49.320 | 3.140 |
High resolution limit [Å] | 2.957 | 2.960 |
Rmerge | 0.160 | 0.735 |
Rpim | 0.072 | 0.363 |
Number of reflections | 17684 | 448 |
<I/σ(I)> | 8.7 | 0.6 |
Completeness [%] | 75.2 | 11.7 |
Redundancy | 6.7 | 5.8 |
CC(1/2) | 0.999 | 0.702 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 7 | 298 | 17% PEG3000, 0.2 M CaAcetate, 0.1 M Tris |