6V7U
Structure of a phage-encoded quorum sensing anti-activator, Aqs1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | CLSI BEAMLINE 08ID-1 |
| Synchrotron site | CLSI |
| Beamline | 08ID-1 |
| Temperature [K] | 105 |
| Detector technology | CCD |
| Collection date | 2016-07-19 |
| Detector | RAYONIX MX-300 |
| Wavelength(s) | 0.9796 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 66.410, 66.410, 73.300 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 45.250 - 2.080 |
| R-factor | 0.2156 |
| Rwork | 0.213 |
| R-free | 0.25740 |
| Structure solution method | SAD |
| RMSD bond length | 0.006 |
| RMSD bond angle | 0.693 |
| Data scaling software | XDS (1.14_3260) |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.14_3260) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.250 | 2.289 |
| High resolution limit [Å] | 2.080 | 2.080 |
| Number of reflections | 12983 | 1290 |
| <I/σ(I)> | 13.81 | |
| Completeness [%] | 99.5 | 99.3 |
| Redundancy | 10.8 | |
| CC(1/2) | 0.998 | 0.500 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 293 | 1M NaH2PO4/K2HPO4, pH 5.6 and 15% glycerol |
