6V7S
Crystal structure of K37-acetylated SUMO1 in complex with phosphorylated PIAS-SIM2
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | CHESS BEAMLINE F1 |
Synchrotron site | CHESS |
Beamline | F1 |
Temperature [K] | 100 |
Detector technology | PIXEL |
Collection date | 2018-03-12 |
Detector | DECTRIS PILATUS3 S 6M |
Wavelength(s) | 0.9775 |
Spacegroup name | P 21 21 21 |
Unit cell lengths | 38.294, 38.457, 120.947 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 23.731 - 1.470 |
R-factor | 0.1722 |
Rwork | 0.171 |
R-free | 0.19250 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 6uyo |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHASER |
Refinement software | PHENIX (1.13-2998_1496) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 23.731 | 1.523 |
High resolution limit [Å] | 1.470 | 1.470 |
Number of reflections | 29982 | 2009 |
<I/σ(I)> | 10.84 | |
Completeness [%] | 95.9 | |
Redundancy | 5.7 | |
CC(1/2) | 0.998 | 0.378 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, HANGING DROP | 298 | 100 mM sodium cacodylate pH 6.5, 19 to 31 % (w/v) PEG3350 and 10 mM calcium chloride. |