6V7P

Crystal structure of SUMO1 in complex with PIAS-SIM2

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSLS BEAMLINE X25
Synchrotron site	NSLS
Beamline	X25
Temperature [K]	100
Detector technology	PIXEL
Collection date	2013-02-18
Detector	DECTRIS PILATUS 6M
Wavelength(s)	1.10
Spacegroup name	P 1 21 1
Unit cell lengths	34.350, 38.467, 62.745
Unit cell angles	90.00, 102.71, 90.00

Refinement procedure

Resolution	30.604 - 1.395
R-factor	0.1543
Rwork	0.152
R-free	0.18380
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	6uyo
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	PHASER
Refinement software	PHENIX (1.13-2998_1496)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	30.604	1.445
High resolution limit [Å]	1.395	1.395
Number of reflections	30629	2526
<I/σ(I)>	15.38
Completeness [%]	95.3
Redundancy	3.1
CC(1/2)	0.998	0.822

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		298	100 mM sodium cacodylate pH 6.5, 19 to 31 % (w/v) PEG3350 and 10 mM calcium chloride.