6V6H

Crystal structure of histidine ammonia-lyase from Trypanosoma cruzi

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	LNLS BEAMLINE W01B-MX2
Synchrotron site	LNLS
Beamline	W01B-MX2
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-09-24
Detector	DECTRIS PILATUS 2M
Wavelength(s)	1.458
Spacegroup name	P 21 21 21
Unit cell lengths	87.812, 144.555, 173.762
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	32.314 - 2.550
R-factor	0.1805
Rwork	0.179
R-free	0.21660
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	1gkm
RMSD bond length	0.003
RMSD bond angle	13.213
Data reduction software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	111.130	2.630
High resolution limit [Å]	2.550	2.550
Number of reflections	72424	6307
<I/σ(I)>	11.9
Completeness [%]	99.3
Redundancy	6
CC(1/2)	0.991	0.732

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		291.15	1 mol L-1 succinic acid, 1% (w/v) PEG mme 2.000 and 0.1 mol L-1 HEPES buffer pH 7.0